Fig. 3.

Inhibition of influenza virus entry into MDCK cells by KAA-2. MDCK cells grown on glass coverslips were infected with A/Oita/OU1 P3-3/09 (H1N1) in the presence or absence of KAA-2 or Amantadine. After 24-h infection, the cells were fixed with 80% acetone for 5 min. Viral antigens in the infected cells were observed under a fluorescence microscope after treatment with an anti-HA mouse monoclonal antibody followed by FITC-conjugated goat anti-mouse IgG. Nuclei within the cells were stained with DAPI (200× magnification).