Fig. 2.

MCPIP1 negatively regulates the IFNβ expression activated by RIG-I, STING, TBK1, IRF3. a HEK293T cells were co-transfected with the plasmids which expressing MCPIP1 and IFNβ-Luc. RIG-IN, STING, TBK1, IRF3, IFN7, IPS-1 or IKKε was co-transfected to activate IFN expression pathway. Twenty-four hours later, the cells were harvested and subjected to a Dual-luciferase assay. Data were presented as mean ± SEM, n = 3. *p < 0.05. b HEK293T cells were respectively co-transfected with the plasmids which expressing MCPIP1 and RIG-I, STING, IPS-1, TRAF3, TBK1 or IKKε. Twenty-four hours later, the cells were harvested and Co-IP detection was assayed.