Fig. 4.

MCPIP1 disrupts TRAF3-TBK1-IKKε interaction. a, b HEK293T cells were respectively co-transfected with the plasmids which expressing MCPIP1, IFNβ-Luc, TRAF3 and TBK1. Twenty-four hours later, the cells were harvested and subjected to Co-IP (a) or Dual-luciferase (b) assay. c, d HEK293T cells were respectively co-transfected with the plasmids which expressing MCPIP1, IFNβ-Luc, TRAF3 and IKKε. Twenty-four hours later, the cells were harvested and subjected to Co-IP (c) or Dual-luciferase (d) assay. Data were presented as mean ± SEM, n = 3. *p < 0.05.