Table 2. Antibodies and the sources used for western blot and immunocytochemical analysis.
Antibody | Species | Company and Catalog # | Dilutions |
---|---|---|---|
FLAG | Rabbit/mouse | Abcam ab1162, ab49763 | 1:5000 (WB) 1:500 (IF) |
GFP | Chicken/mouse | Abcam ab13970, ab1218 | 1:1000 (WB) 1:1000 (IF) |
VP16 | Rabbit | Abcam ab4808 | 1:1000 (WB) 1:250(IF) |
Beta-Tubulin | Rabbit/chicken | Abcam ab18207, Abcam ab18207 | 1:10,000 (WB) |
AT180 | Mouse | Thermo Scientific, MN1040 | 1:5000 (WB) 1:500(IF) |
AT8 | Mouse | Thermo Scientific, MN1020 | 1:5000 (WB) 1:500(IF) |
GAPDH | Mouse | Millipore, CB1001-500UG | 1:20,000 (WB) |
Tau12 | Mouse | Abcam, ab74137 Millipore, MAB2241 | 1:20,000 (WB) |