Table 1. Read statistics for Illumina and Nanopore sequencing runs.

	L. virgatum Illumina	L. vulgare Illumina	L. vulgare Nanopore
raw reads (300+300 run / 80+80 run)	381,932 (25,290 / 356,642)	373,518 (22,810 / 350,708)	52,223
PhiX spike-in reads removed (%)	2,620 (0.7)	5,560 (1.5)	n.a.
quality- and length-filtered reads (%)	167,126 (43.8)	119,502 (32.0)	217 (0.4)
remaining reads for analysis (%)	212,186 (55.6)	248,456 (66.5)	52,006 (99.6)

Number of raw reads is given for each of both MiSeq 300-bp and 80-bp paired-end sequencing runs; for Nanopore it refers to reads after processing by Porechop. Length-filtered reads include those which were too short after trimming. Read numbers for Nanopore filtered reads as well as remaining reads refer to processed, not fully processed reads (for information on those see text).

L., Leucanthemum; n.a., not applicable.