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. 2020 Mar 24;15(3):e0230358. doi: 10.1371/journal.pone.0230358

Fig 1. Proximity-ligation assay (PLA) schematic and lectin specificity.

Fig 1

Biotinylated lectins with indicated specificities were added to first label specific sugars. Then, anti-ICAM-1 antibody and avidin, conjugated to complementary oligos, were added. When lectin-recognized sugar epitopes are less than 40 nm from anti-ICAM-1, the complementary oligos hybridize and amplify, producing red fluorescent puncta. Right panel shows N-glycan structures recognized by different lectins used (adapted from [19]. Man = Mannose; Gal = Galactose; GlcNac = N-acetylglucosamine; Sa = Sialic acid; R = varying N-glycan structures.