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. 2020 Feb 28;11:307. doi: 10.3389/fmicb.2020.00307

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Copyright © 2020 Khoury, Vila, Puthran, Sultan, Montelongo-Jauregui, Melo and Jabra-Rizk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal Laser Scanning Microscopy (CLSM) analysis of S. mutans biofilms grown in C. albicans cell free spent media or media supplemented with purified extracellular matrix. (A) 24-hr single biofilms of GFP-S. mutans appeared sparse with minimal cells or cell aggregates seen. (B) Significantly more S. mutans cells was seen when S. mutans was grown in C. albicans spent media with considerable amount of bacterial clumping around secreted polysaccharide aggregates. (C) S. mutans biofilm grown in the presence of purified extracellular matrix from C. albicans biofilms showing large aggregates of polysaccharides with embedded aggregates of bacteria. Pink: polysaccharides stained with Con-A; Green: GFP-S. mutans.