FIG 1.

Survival of isolates following phagocytosis by RAW 264.7 and THP-1 macrophages, assessed by gentamicin protection assay. (A) Survival of isolates at 2, 4, 8, and 24 hours postinfection (hpi) following phagocytosis by RAW 264.7 macrophages and at 2, 4, 8, 24, and 168 hpi following phagocytosis by THP-1 macrophages, reported as log₁₀ CFU/ml. (B) Survival of isolates at 4, 8, and 24 hpi following phagocytosis by RAW 264.7 macrophages and at 4, 8, 24, and 168 hpi following phagocytosis by THP-1 macrophages, reported as log₂ fold change compared to survival at 2 hpi. Results for isolates in the study correspond to the mean of three independent assays (n = 3) with duplicate technical replicates. Results for reference Salmonella enterica serovar Typhimurium strains 14028S and ST4/74 correspond to the mean of six independent assays (n = 6) with duplicate technical replicates. (C) Survival at 2, 4, 8, 24, 48, 72, 96, 120, 144, and 168 hpi of S. enterica Tennessee CFSAN001387, in comparison with S. Typhimurium ST4/74, following phagocytosis by THP-1 macrophages, reported as log₁₀ CFU/ml. (D) Survival at 4, 8, 24, 48, 72, 96, 120, 144, and 168 hpi of S. Tennessee CFSAN001387, in comparison with S. Typhimurium ST4/74, following phagocytosis by THP-1 macrophages, reported as log₂ fold change compared to survival at 2 hpi. Results correspond to the mean of three independent assays (n = 3) with triplicate technical replicates. The lower and upper hinges correspond to the 25th and 75th percentiles, with the whiskers extending ±1.5 times the range between first and third quartiles.