Monitoring live organism shedding from mice coinoculated with both plasmid-free and wild-type chlamydial organisms. CBA/J (n = 5) intravaginally inoculated with Pf C. muridarum (clone CMUT3.5) were intragastrically coinoculated with Wt Chlamydia (CMpmCherry) 7 days after intravaginal inoculation (see the Fig. 4 legend above). On day 28 after intravaginal inoculation, mice were sacrificed for recovering live organisms (black bar for Pf and red bar for Wt log10 IFU along the y axis) from both genital (vagina/cervix [V], uterine horn [UH], and oviduct and ovary [O]) and GI (stomach, duodenum, jejunum, ileum, cecum, colon, and rectum) tissues (x axis).