FIG 3.

Inhibition of tRNA synthetase results in morphological aberrance in C. trachomatis L2. Representative images of HEp-2 cells infected with C. trachomatis and treated or not as indicated. Cells were fixed and stained 24 hpi using primary antibodies to major outer membrane protein (MOMP) and chlamydial Hsp60_1. Treatments of 120 μM indolmycin and 1 μg·ml⁻¹ AN3365 given at 10 hpi resulted in smaller inclusions and morphologically aberrant organisms similar to IFN-γ-treated organisms. All images were acquired on Zeiss LSM 800 confocal microscope with Airyscan at 63× optical magnification with 2× digital zoom. Bars, 5 μm.