FIG 7.

Transcriptional changes in Chlamydia consistent with persistence can be detected during indolmycin and AN3365 treatment. (A) Transcripts of euo were elevated in C. trachomatis (Ctr) or C. pneumoniae (Cpn) following treatments with 120 μM indolmycin, 1 μg·ml⁻¹ AN3365, or IFN-γ. (B) Under standard conditions, groEL_1 transcripts in C. trachomatis decreased between 10 and 24 hpi. However, treatment with indolmycin resulted in unchanged transcript levels, similar to what is seen in IFN-γ-treated samples, while AN3365 treatment resulted in slightly higher transcript levels (P = 0.053). In C. pneumoniae, no significant change was seen between 24 and 48 h under any treatment, in agreement with previous reports investigating IFN-γ exposure. (C) trpB transcripts accumulated at 14 hpi, as expected, in indolmycin- and IFN-γ-treated samples but not in those treated with AN3365. At 24 hpi, AN3365-treated samples exhibit a slight (3- to 4-fold) increase in trpB (P = 0.076). Student's t test was used to compare each 24 hpi value to that at 10 hpi untreated in C. trachomatis and each 48 hpi value to that at 24 hpi untreated in C. pneumoniae following Log₁₀ transformation. *, P < 0.05.