TABLE 2.
No. of days postinfection | Mouse survival rate (%)d
|
||
---|---|---|---|
Nontransfer | Naive control | Immunized | |
T cell | |||
7 | 100 | 100 | 100 |
14 | 100 | 100 | 100 |
21 | 100 | 100 | 100 |
28 | 80 | 80 | 100 |
35 | 60 | 60 | 100 |
60 | 60 | 60 | 100b ,c |
NK cell | |||
7 | 100 | 100 | 100 |
14 | 100 | 100 | 100 |
21 | 100 | 100 | 100 |
28 | 80 | 80 | 100 |
35 | 60 | 60 | 100 |
60 | 60 | 60 | 100b ,c |
Serum | |||
7 | 100 | 100 | 100 |
14 | 100 | 100 | 100 |
21 | 100 | 100 | 100 |
28 | 80 | 80 | 100 |
35 | 60 | 60 | 100 |
60 | 60 | 60 | 100b ,c |
Serum and NK cell | |||
7 | 100 | 100 | 100 |
14 | 100 | 100 | 100 |
21 | 100 | 100 | 100 |
28 | 80 | 80 | 100 |
35 | 60 | 60 | 100 |
60 | 60 | 60 | 100b ,c |
B cell | |||
7 | 100 | 100 | 100 |
14 | 100 | 100 | 100 |
21 | 100 | 100 | 100 |
28 | 80 | 80 | 100 |
35 | 60 | 60 | 100 |
60 | 60 | 60 | 100b ,c |
Several types of immune cells and serum from GFP-DDDHA strain-immunized mice were given to naive C57BL/6 mice (aged between 10 and 14 weeks) by adoptive transfer. Mice without adoptive transfer or with naive control adoptive transfer were used for comparison (see Materials and Methods for details). After 24 h of postadoptive transfer, mice were infected with 5 × 103 WT Tulahuen trypomastigotes, which is sublethal for 10- to 14-week-old C57BL/6 mice. Survival rates were documented through the acute phase to the subacute phase.
P < 0.01 compared to nontransfer mice.
P < 0.01 compared to mice that received the naive control.
The survival rates of mice were analyzed by unpaired two-tailed t test to determine the statistical significance between transferred mice, nontransfer mice and mice with naive control. n = 5 to 10. Data presented are one representative example of four separate experiments. All four experiments produced similar results.