TABLE 1.
Functional antibodies generated by the chimeric OspA variants, as determined in serum bactericidal assaysa
| Immune serum | SB titer forb
: |
|||||
|---|---|---|---|---|---|---|
| ST1 | ST2 | ST3 | ST4 | ST5 | ST6 | |
| FL-OspA | 160 | 2,500 | 2,500 | 160 | 12,500 | <20 |
| V2 | 160 | <20 | <20 | 80 | 2,500 | 500 |
| V3 | 160 | <20 | <20 | 20 | 500 | 500 |
| V5 | <20 | 2,500 | <20 | 160 | 20 | 62,500 |
| V6 | <20 | 500 | <20 | 320 | <20 | 500 |
a
Serum bactericidal assay was performed with B. burgdorferi ZS7 (ST1), B. afzelii LU171 (ST2), B. garinii PFr (ST3), B. bavariensis DK6 (ST4), B. garinii PHei (ST5), and KL11 (ST6).
b
Serum bactericidal (SB) titer is defined as the reciprocal of the lowest dilution showing ≥50% reduction in spirochetes compared to negative sera.