FIG 2.

Antibiotic-resistant bacterial strains exhibit distinct capacities to disseminate to mLNs and to recruit neutrophils to the colonic lamina propria. (A) Wild-type C57BL/6 mice (n, 4 to 5 per group) were treated with metronidazole, vancomycin, and neomycin (MNV) for 3 days, followed by ampicillin for 4 days. Then the mice were inoculated with 2 × 10⁴ CFU of 1 of 12 antibiotic-resistant bacterial strains. Ampicillin was maintained in the drinking water for an additional 2 days. (B) Five days following inoculation, cells isolated from the colonic lamina propria were profiled by flow cytometry. (C) Bacterial dissemination to mLNs was determined by selective plating of whole mLNs.