FIG 2.

Endogenous antibiotic degradation impacts treatment of C. difficile infection. (A) Schematic representation of the experimental design. (B) Reconstitution levels for mice reconstituted with either WT or mut BLA E. coli (as depicted in panel A) at 1 day after oral gavage, as assessed by selective plating of fecal pellets (n = 14; data are pooled from two independent experiments; individual data points and geometric means are shown). (C) C. difficile burdens in mice treated as depicted in panel A at 1 day after oral gavage, as assessed by selective plating of fecal pellets (n = 14; data are pooled from two independent experiments; individual data points and geometric means are shown). (D) C. difficile burden in the cecal content of mice treated as depicted in panel A at day 3 postinfection (n = 7 for controls; n = 12 for ampicillin treated). Data are pooled from three independent experiments. Individual data points and geometric means are shown (Kruskal-Wallis test with multiple comparisons: *, P < 0.05; **, P < 0.01).