Table 2. Statistic analysis of the raw sequencing data and clean data.
Sample | Raw_reads | Clean_reads | GC_rate(%) | Q20(%) | Q30(%) | Rate(%) |
I-1 | 144,423,710 | 133,047,782 | 46.63 | 95.31 | 85.19 | 92.12 |
I-2 | 124,706,334 | 114,120,378 | 46.47 | 94.81 | 83.80 | 91.51 |
II-2 | 151,862,788 | 140,826,868 | 46.35 | 95.67 | 86.15 | 92.73 |
II-3 | 106,989,394 | 98,095,844 | 44.92 | 95.03 | 84.39 | 91.69 |
II-5 | 171,312,630 | 159,937,778 | 46.72 | 95.91 | 86.77 | 93.36 |
III-1 | 150,264,330 | 139,860,546 | 47.95 | 95.79 | 86.52 | 93.08 |
III-2 | 128,088,996 | 118,490,886 | 47.43 | 95.46 | 85.64 | 92.51 |
III-3 | 133,805,476 | 123,609,822 | 48.41 | 95.61 | 86.07 | 92.38 |
Note: Paired-end reads were generated with BGISEQ-500 platform, then the reads with sequencing adapters, N base, poly base, low quality etc. were filtered out with SOAPnuke.