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Schematic of microfluidic setups used to generate gradients of soluble signaling factors across MIXL1-GFP hPSCs. Cells undergoing differentiation into anterior primitive streak (PS) were observed with live-cell fluorescence microscopy. (a) Static Ibidi -slide chemotaxis microfluidic device. (b) Flow-based Y-channel polydimethylsiloxane (PDMS) microfluidic device.
