FIG. 2.

comsol multiphysics numerical simulation of extracellular signal concentrations across the cell culture chamber in the Ibidi static microfluidic device. (a)–(d) Graphs of the distribution of primitive streak (PS) soluble signaling factors in the Ibidi static microfluidic device. (e) and (f) Comparison of the establishment of the gradient between small molecule WNT agonist CHIR99021 and protein BMP4, where the color map indicates the concentration of the respective morphogen. The center rectangle depicts a top-down view of the central cell chamber. Scale bar: 1 mm.