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Time-lapse bright-field and fluorescence imaging of primitive streak (PS) induction in MIXL1-GFP hPSCs in a static Ibidi microfluidic device. Differentiation in cell colonies begins on the left side of the cell culture chamber due to the higher concentration of primitive streak-inducing signals. Cell morphology changes over time in response to CHIR99021 exposure. Fluorescent green (GFP) indicates MIXL1 expression and thus the induction of primitive streak. Scale bar: .
