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Concentration profiles of extracellular signals vary as a function of flow rate in Y-channel microfluidic devices. (a) COMSOL simulation of a gradient of the small molecule WNT agonist CHIR99021 at various flow rates. (b) TMR concentration gradient profiles as a function of flow rate in a 2 mm wide Y-channel microfluidic device. The gradient appears sharper as the flow rate in the channel is increased. In micrographs at right, TMR appears gray, PBS appears black. Scale bar: .
