FIGURE 3.

P2Y₁₂ inhibition reduces silicotic lung inflammation and fibrosis. Representative lung parenchyma photomicrographs 14 days after PBS (A) or silica (20 mg) injection without (B) or with (C) clopidogrel treatment (20 mg/kg) (CTRL, SIL, and SIL + Clopi groups, respectively). Hematoxylin-eosin staining (left and central columns) and picrosirius staining (right column). (D) Quantification of nodular area, neutrophil infiltration, and collagen fibers (left, central, and right columns, respectively). Box plots show median values of 3–5 animals/group (16–20 images/animal) with respective minimum to maximum values. *p < 0.05 in relation to silica (SIL). Arrows show inflammatory infiltrates. Bars: 1000 μm (left column), 50 μm (central and right columns).