Figure 3.

Introduction of gradient cues into various hydrogel systems based on the BSNF blocks. (A) NIPAM-based hydrogels with mechanical gradient cues: (a) macrographic images of NIPAM hydrogels with mechanical gradient cues, (b) The gradient and anisotropic mechanical properties of the hydrogels, (c) FTIR spectral of the hydrogels at different areas; (B) Gel-MA-based hydrogels with mechanical gradient cues: (a) macrographic images of Gel-MA hydrogels with mechanical gradient cues, (b) The gradient and anisotropic mechanical properties of the hydrogels, (c) FTIR spectral of the hydrogels at different areas; (C) Silk nanofiber hydrogels with rhodamine gradients: (a) macrographic images of silk hydrogels with rhodamine gradients, (b) Confocal microscopy images of different areas of rhodamine-loaded hydrogels, (c) Rhodamine fluorescence intensity of different areas of rhodamine-loaded hydrogels. The samples were as follows: MGH-N, NIPAM-BSNF composite hydrogels with gradient cues; MGH-M, GEL-MA-BSNF composite hydrogels with gradient cues; MGH-R, rhodamine-loaded ASNF-BSNF composite hydrogels with gradient cues