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. 2020 Feb 12;11(4):267–285. doi: 10.1007/s13238-020-00692-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cytocompatibility and cell adhesion on the hydrogels with mechanical cues. (A) Confocal microscopy images of BMSCs on the hydrogels when cultured for 1, 6, and 12 days; (B) BMSC proliferation on different hydrogels when cultured for 1, 3, 6, 9 and 12 days; (C) immunofluorescence staining of adhered BMSCs on the hydrogels for 24 h. Nuclei is stained in blue. Vinculin is stained in red, and F-actin is stained in green. The white arrows indicate the oriented spreading of cells. ns means none statistically significant