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. 2020 Mar 24;11:1539. doi: 10.1038/s41467-020-15344-3

Fig. 4. The apoptotic factor in culture supernatant from bacteria is a low molecular weight protein.

Fig. 4

a, b Culture supernatant from bacteria was separated into fractions of <10 kDa and >10 kDa by filtration and each fraction was added to A549 alveolar epithelial cells after 1/10 dilution to determine apoptosis by flow cytometry. For each group, n = 3. Bars indicate the means ± S.D. Statistical analysis by ANOVA and Tukey’s test. *p < 0.001 vs medium control. LMW low molecular weight, HMW high molecular weight. The source data underlying b are provided in the Source Data file.