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. 2020 Mar 24;10:5315. doi: 10.1038/s41598-020-62014-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TMP MIC values for E. coli expressing Bd DHFR substituted variants. Measurements were performed in 96-well plates as quadruplicates, using MHII broth supplemented with 50 µg/mL kanamycin and 0.01 mM IPTG. Plates were incubated at 37 °C for 20 hours under shaking conditions. MIC values were calculated by fitting data to a modified Gompertz equation¹⁵. Error bars represent 95% confidence intervals.