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. 2020 Mar 24;10:5343. doi: 10.1038/s41598-020-61683-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry of lungs 24, 48, and 72 hrs following IPL saline (A,C, and E) and IPL CpG-ODN (B,D, and F) delivery, respectively. At magnification 20×, the CpG-ODN treated lung showed substantially high infiltration of CD3⁺ T cells (brown color) compared to the saline control lung at 24 hr (B vs. A), 48 hr (D vs. C), and 72 hr (F vs. E) post treatment.