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. 2020 Mar 24;10:5343. doi: 10.1038/s41598-020-61683-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pro-Inflammatory cytokine profiles in lungs and spleen. The luminescence intensity of each gene was normalized to the averages of HKGs (Hprt 1 and Tubb 1). Fold changes were calculated against the control at each time point. (Broken lines indicate the fold change of one, which shows no change compared to the control group). Sidak multiple comparisons test following ANOVA was used to analyse the significance of gene expression between CpG-ODN and the saline control group at each time point. Asterisks indicate the fold changes that were significantly different (P < 0.05).