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. 2020 Mar 24;11:1531. doi: 10.1038/s41467-020-15270-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Scheme of experimental design for studying the conformations of VAMP2 in cholesterol-level-regulated cells. Treatments 1 and 2 for increasing and decreasing cellular cholesterol levels were noted in the Methods section. b Overlay of 2D ¹H-¹⁵N NMR spectra of VAMP2(1–96) in cholesterol up-regulated (Chol-up, blue), down-regulated (Chol-down, red) and control (Ctrl, black) HEK-293T cells at the same contour levels. Representative crosspeaks were enlarged on the right. c Relative NMR signal intensity changes of VAMP2(1–96) in Chol-up (blue) and Chol-down (red) cells to those in control cells. Details of data processing are described in the Methods section. Source data are provided as a Source Data file.