Fig. 4. VAMP2 SNARE motif sensors the electrostatic property of membrane surface.

a Residue-resolved NMR signal intensity ratios (I/I₀) of VAMP2(1–96) titrated by acidic liposomes (DOPS, DOPG, liver PI, and egg PA) to that in solution at indicated lipid/protein molar ratios. b Residue-resolved NMR signal intensity ratios (I/I₀) of VAMP2(1–96) titrated by neutral liposomes (DOPC, cholesterol/DOPC (1/1, mol/mol) and DOPE/DOPC (1/1, mol/mol)) to that in solution at indicated lipid/protein molar ratios. c Comparisons of individual lipid classes in lipid-raft and non-raft SV. Error bars are standard deviations from three biological replicates. The data were analyzed by Student’s t-test. The p-values of all lipid subclasses, except for PC and PA, are less than 0.001. n.s. represents not significant. * indicates p-value < 0.05. d Schematic presentation of VAMP2 extravesicular domain on lipid-raft and non-raft membranes. Source data are provided as a Source Data file.