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. 2020 Mar 24;10:5356. doi: 10.1038/s41598-020-62202-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of integrin α6 but not of integrin α3 strongly inhibits PsV binding. Two days after siRNA transfection, cells were incubated for 1 h at 0 °C with HPV16 PsVs, scraped from the substrate, washed, lysed and analyzed by Western blot. Full Western blots are shown in Fig. S16 (L1) and S17 (actin). Binding of viral particles was assayed by quantification of the L1 ~55 kDa band. The control value was set to 100% and used for normalization. Values are given as means ± SD (n = 4 independent experiments). Unpaired Student’s t-test, comparing control to knockdown conditions (***p < 0.001; *p < 0.05).