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. 2020 Mar 24;10:5320. doi: 10.1038/s41598-020-62074-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glycosphingolipid (GSL)-glycan MS profiles of human lung following neuraminidase treatment. All molecular ions detected represent reduced, permethylated species and are present in the form of [M + Na]⁺. (a) MALDI-TOF-MS spectrum of untreated human lung GSL-glycans. (b) MALDI-TOF-MS spectrum of human lung GSL-glycans treated with neuraminidase S. (c) MALDI-TOF-MS spectrum of human lung GSL-glycans treated with neuraminidase A. Peaks representing sialylated glycans are colored in red and non-sialylated glycans are in black. Symbol representations of monosaccharides are: yellow circle, Galactose; red triangle, Fucose; blue square, N-acetylglucosamine; half-blue/half-yellow square, N-acetylhexosamine; purple diamond, N-acetylneuraminic acid.