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. 2020 Mar 24;10:5335. doi: 10.1038/s41598-020-62091-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Abnormal frequency of Spaca6 KO sperm presence in the perivitelline space. After cumulus-intact IVF using sperm from Spaca6+/+, Spaca6+/− or Spaca6−/− C57BL/6 males, were reported: (a) the percentage of perivitelline space sperm containing-oocytes (mean ± SEM) which showed high and significant difference when comparing the KO to heterozygotes or WT groups (P < 0.0001) and less but significant difference when comparing heterozygotes and WT groups (P < 0.05), or (b) sperm number in perivitelline space per oocyte (mean ± SEM). In this case, only the difference between KO sperm and the two other groups was significant (P < 0.0001).