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. 2020 Mar 24;10:5307. doi: 10.1038/s41598-020-62131-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Classification trees obtained using all samples for Visit 1 (A) Visit 3 (B). In a tree, unfilled circles represent nodes and solid circles represent terminal leaves. Each node shows the name of the metabolite and the rule for splitting. Each leaf, which is termed “case” or “control” based on the rule, lists the number of cases and controls that were classified to the leaf in the CART procedure and the corresponding posterior probability.