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. 2020 Mar 24;10:5313. doi: 10.1038/s41598-020-62140-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of hADSC-EVs to HUVECs on the proliferation, migration and tube formation on Matrigel. (A) Image and (B–D) semi-quantitative EdU, transwell migration and tube formation assays of HUVECs with and without hypoxic hADSC-EVs or hADSC-EVs. The proliferation, migration and mean tube structure of HUVECs treated with hADSC-EVs or hypoxic hADSC-EVs were higher than those in the control group. Moreover, the ability of proliferation, migration and tube formation of HUVECs could be further enhanced by treatment with hypoxic hADSC-EVs (compared with normal hADSC-EVs). Data are presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01. Scale bar in EDU test: 100 μm. Scale bar in transwell and tube formation assays: 1 mm.