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. 2020 Mar 24;10:5313. doi: 10.1038/s41598-020-62140-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Let-7/AGO1/VEGF signalling pathway detection. (A) The results of qRT-PCR for let-7 families in hADSCs, hypoxic hADSCs and LIN28B transfected hADSCs. (B) The results of qRT-PCR for let-7 families in hADSC-EVs, hypoxic hADSC-EVs and LIN28B transfected hADSC-EVs. (C) The results of qRT-PCR for let-7 families in HUVECs treated by hADSC-EVs, hypoxic hADSC-EVs and LIN28B-hADSC-EVs. (D) The results of qRT-PCR for AGO1 in HUVECs treated by hADSC-EVs, hypoxic hADSC-EVs and LIN28B-hADSC-EVs. (E) The results of qRT-PCR for VEGF in HUVECs treated by hADSC-EVs, hypoxic hADSC-EVs and LIN28B-hADSC-EVs. (F) The results of ELISA assay for VEGF secretion of HUVECs treated by hADSC-EVs, hypoxic hADSC-EVs and LIN28B-hADSC-EVs. Data are presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01.