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. 2020 Mar 24;10:5313. doi: 10.1038/s41598-020-62140-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The histological analysis of fat grafts (n = 12). (A,D) The results of CD31(green)/human mitochondria immunofluorescence(red) staining indicated that, compared with grafts co-transplanted with hADSC-EVs, grafts co-transplanted with LIN28B-hADSC-EVs exhibited decreased exogenous neovascularisation counts at post-grafting week 4. however, the count of exogenous neovascularization of LIN28B-hADSC-EVs co-transplanted grafts were still higher than them in control grafts. (B,E) The results of immunofluorescence staining (human mitochondria) indicated that, compared with grafts co-transplanted with hADSC-EVs, grafts co-transplanted with LIN28B-hADSC-EVs performed and decreased retention rate of exogenous adipose tissue at post-grafting week 12. Of note, the retention rate of exogenous adipose tissue of LIN28B-hADSC-EVs co-transplanted grafts were still higher than those in control grafts. (C,F) The results of Ki-67(green)/human mitochondria immunofluorescence(red) staining indicated that, compared with control grafts, grafts co-transplanted with hADSC-EVs exhibited increased Ki-67 positive cells in grafts at post-grafting week 1 (P < 0.05), and grafts co-transplanted with hypoxic hADSC-EVs exhibited more Ki-67 positive cells in grafts compared with hADSC-EVs treated grafts at the same time point (P < 0.05). Moreover, decreased number of Ki-67 positive cells of LIN28B-hADSC-EVs co-transplanted grafts were detected compared with hADSC-EVs co-transplanted grafts (P < 0.05). However, the number of Ki-67 positive cells of LIN28B-hADSC-EVs co-transplanted grafts were still higher than that in control grafts (P < 0.05). Data are presented as the mean ± SD (n = 12). *P < 0.05, **P < 0.01. Scale bar: 200 μm.