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. 2020 Mar 24;10:5362. doi: 10.1038/s41598-020-62071-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Experimental design of blended actions session (Session 1). Each run consisted of 6 blocks of 38 s with IBI of 12 s. After 20 s fixation, subjects started to simultaneously perform action observation and motor imagery (AO+MI). In each block, 14 video trials of 2000ms each were presented with a variable ISI from 500–1000 ms. (B) Experimental block design of natural actions session (Session 2). Each run contained 9 chunks (gray shaded area), and there were 3 chunks for each action. A chunk comprised an AO+MI block with 8 repetition of the same action video of 16 s, a short IBI of 4s, a pure MI block of 16 s and a long IBI of 12 s. After 20s fixation, participants observed and imagined (AO+MI) doing an action simultaneously for 8 times then imagined (MI) it 8 times assisted by a pacing tone⁵⁰.