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. 2020 Mar 24;10:5259. doi: 10.1038/s41598-020-61015-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intracellular SINV RNA levels in BHK cells are increased by higher MOI and by anti-E2 antibody treatment. Time-lapse live-cell imaging (A), objective lens 20X) of TE-UTR-4Br-infected BHK cells (3–5.5 h after infection) on the same settings shows the effects of multiplicity of infection (10 vs. 100) and anti-E2 [SV127] antibody treatment (5 μg/ml, 2 h after infection) on the accumulation of viral RNA (green). The graph (B) shows the average signal intensity of the 5 brightest cells from each group (1 frame = 150 seconds). Scale bar = 100 μm.