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. 2020 Mar 24;10:5259. doi: 10.1038/s41598-020-61015-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibody- and IFNγ-mediated SINV RNA clearance from dAP7 cells. Live-cell imaging (objective lens 20X, lambda mode) shows the effects of anti-E2 antibody and IFNγ on viral RNA clearance in TE-UTR-4Br-infected dAP7 cells (MOI = 20) at 12, 24, 48 and 72 h after infection (yellow – autofluorescence; green – Broccoli-DFHBI-1T; red – propidium iodide (dead cells)). All treatments (TrkA control antibody 5 μg/ml; anti-E2 5 μg/ml, rat IFNγ 500 U/ml) were applied 2 h after infection. Scale bar = 100 μm.