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. 2020 Mar 18;11:379. doi: 10.3389/fmicb.2020.00379

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Copyright © 2020 Wang, Mu, Xie, Cheng, Fu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The biological characteristics and dsRNA pattern of Sclerotinia sclerotiorum strain PX14A4. (A) Colony morphology of strain PX14A4 and virulent strain Ep-1PNA367 (on PDA for 14 days at 21°C) (top). Lesion induced by strain PX14A4 or Ep-1PNA367 on detached rapeseed leaves (72 h post inoculation under 21°C) (bottom). Growth rates (B) and average lesion diameters (C) of strains PX14A4 and Ep-1PNA367. “**” indicates a significant difference (p < 0.01) between strain PX14A4 and Ep-1PNA367. (D) Agarose gel electrophoresis analysis of the dsRNA extracted from strain PX14A4. The dsRNA was treated with DNase I and S1 nuclease prior to electrophoresis. The dsRNA was named 8K, 2.5K, 1.8K, and E. Size of the DNA ladder standard is indicated in base pair (bp). M, molecular weight marker (Takara).