FIGURE 6.

Detection of synthesized SsOLV4 RNA in the strains transfected using mycelia as the receptor. As described in the section “Materials and Methods,” the linearized vector pMD18-P1 was used to synthesize SsOLV4 RNA. The synthesized viral RNA was mixed with PEG buffer and added onto tips of fresh mycelia of strain N69. The infection experiments were performed on 21 colonies of strain N69. Each colony treated (named as T1 to T21) was subcultured three times (for about 10 days). The total RNA of all strains obtained was isolated to detect SsOLV4 using RT-PCR (A) and dot blot (B). The RT-PCR primers and dot hybridization probe were the same as used in Figure 5. Total RNAs of strains M6 and N69 were used as positive and negative controls, and water (H₂O) was used as a blank control. In (B), the parallelism strain name was listed under the dot hybridization result. Strains infected with SsOLV4 were indicated in red.