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. 2020 Mar 18;11:379. doi: 10.3389/fmicb.2020.00379

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Copyright © 2020 Wang, Mu, Xie, Cheng, Fu and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biological properties of the S. sclerotiorum strain artificially infected by SsOLV4. Strains M6, N39, and N69 are the protoplasm-regenerants of strain PX14A4; strains N39P, N69P, and Ep-1PNA367P are the strains transfected by synthesized RNA. A synthesized virus was also transmitted to Ep-1PNA367 through hyphal anastomosis to obtain strains M6TA, N39PTA, N69PTA, and Ep-1PNA367PTA. (A) Colony morphology of strains (PDA, 14 days, 21°C). (B) Radial mycelial growth rate (PDA, 21°C). (C) Lesion diameter on rapeseed leaves (21°C). In each histogram, bars labeled with the same letters are not significantly different (p > 0.05) according to the least significant difference test.