TABLE 1.
Strains and plasmids used in this study.
| Strains and plasmids | Relevant description | Source |
| Strains | ||
| Escherichia coli DH5α | Host for general cloning | This lab |
| S. spinosa | Spinosad producing strain | This lab |
| S. pogona | Butenyl-spinosad producing strain | This lab |
| SPOG-RM | S. pogona harboring pJNRM | This study |
| SPOG-ME | S. pogona harboringpKCcas9-metK | This study |
| Plasmids | ||
| pMD18-T Vector | E. coli cloning vector, containing AmpR and LacZ | |
| pMDGK | pMD18-T containing gdh+kre | This study |
| pMDGT | pMD18-T containing gtt | This study |
| pMDE | pMD18-T containing epi | This study |
| pMDRM | pMD18-T containing gdh+kre, gtt and epi | This study |
| pMDME | pMD18-T containing metK | This study |
| pJN100 | E. coli-Streptomyces shuttle expression vector | This lab |
| pKCcas9dO | E. coli-Streptomyces shuttle expression vector | This lab |
| pJNRM | pJN100 containing gdh+kre, gtt and epi | This study |
| pKCcas9-metK | pKCcas9dO containing metK | This study |