Table 4.
Medium1 | Treatment2 | Explant survival (%)3 | Explants with bulblet formation (%)3 | No. of bulblet produced3 |
---|---|---|---|---|
Liquid | A | 25.0c | 91.7a1 | 6.4bc |
B | 41.7bc | 91.7a | 7.0bc | |
C | 75.0bc | 91.7a | 3.9c | |
D | 58.3abc | 91.7a | 5.3bc | |
Solid | A | 66.7ab | 100.0a | 11.5b |
B | 66.7ab | 100.0a | 26.7a | |
C | 91.7a | 100.0a | 8.6bc | |
D | 83.3a | 100.0a | 7.2bc |
11/2 X MSBM with 2.5% sucrose and 0.4% GPP. Cultures were incubated at 20 °C. Data were recorded after 2 months of culture. Each treatment had 12 replicates
2A: longitudinal sections; B transverse sections; C: longitudinal incision; D: transverse incision
3Means followed by the different letter of a column are significantly different at 5% level by LSD test