Fig. 1.

Live 22 GSC cells have increased OCR and corresponding increased mean NADH lifetimes and increased amount of bound NADH ($a_2\%$) compared with NSCs. (a) OCR basal measurements taken with Seahorse XF (Agilent) assay by seeding 17 wells with $\mathrm{50,000}\text{\hspace{0.17em}\hspace{0.17em}cells}/\text{well}$. Mean OCR for $22\mathrm{GSCs}=43.04\pm 29.84\mathrm{pMole}/\min$ versus $\mathrm{NSCs}=26.10\pm 7.92\mathrm{pMole}/\min$ (Student’s $t$-test, $p=0.0423$). Error bars represent standard deviations. FLIM measurements taken on same cell lines with custom multiphoton microscope. Mean NADH lifetimes for (b) 22 GSCs $t_{\text{mean}}=1117.39\pm 106.83\mathrm{ps}$, $a_2\%=33.0\pm 3.6\%$ versus (c) NSCs $t_{\text{mean}}=1021.73\pm 40.06\mathrm{ps}$, $a_2\%=30.0\pm 1.5\%$ (Student’s $t$-test, $p=0.005714$). Scale bars are $50\mu \mathrm{m}$.