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. 2020 Mar 13;23(4):100983. doi: 10.1016/j.isci.2020.100983

Figure 4.

Figure 4

Interference of Renin Prohibits IL-17 Productions in OLP Cell Models

(A) Efficacy of siRNA-mediated knockdown on renin expression was detected by Western blot in HOKs.

(B and C) Real-time PCR quantification of IL-17 mRNA levels in HOKs stimulated with activated CD4+ T cells (B) or 100 ng/mL LPS (C) for 8 h, following 36-h scramble- or renin-siRNA pretreatment.

(D and E) ELISA detection of IL-17 secretions in the culture medium from HOKs stimulated with activated CD4+ T cells (D) or 100 ng/mL LPS (E) for 8 h, following 36-h scramble- or renin-siRNA pretreatment.

(F–I) HOKs were transfected with scramble- or p65-siRNA for 36 h, followed by 8-h activated CD4+ T cells or 100 ng/mL LPS treatment. Real-time PCR (F and G) and ELISA (H and I) tests were performed.

∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. corresponding control; #p < 0.05, ##p < 0.01, ###p < 0.001 versus LPS or CD3/28 group, n = 3. Ctrl, control. Data were shown as means ± SD. Two-tailed Student's t test and one-way analysis of variance were used.