Figure 6.

Vitamin D/VDR Signaling Inhibits Renin and IL-17 Transcripts in OLP Cell Models

(A) HOKs were pretreated with different doses of 1,25VD for 12 h, and then challenged by renin plasmids transfection for 36 h

(B) HOKs were co-transfected with VDR and renin plasmids for 36 h.

(C and D) HOKs were pretreated with different doses of 1,25VD for 12 h and then challenged by activated CD4⁺ T cells (C) or 100 ng/mL LPS (D) for 8 h.

(E and F) HOKs were transfected with different doses of VDR plasmids for 36 h, followed by 8-h activated CD4⁺ T cells (E) or 100 ng/mL LPS (F) treatment. Renin and IL-17 transcripts were quantified by real-time PCR.

∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus corresponding control; #p < 0.05, ##p < 0.01, ###p < 0.001 versus LPS or CD3/28 group, n = 3. 1,25VD, 1,25(OH)₂D₃. Data were shown as means ± SD. Two-tailed Student's t test and one-way analysis of variance were used.