Table 1.
Primary Antibodies Used for IHC
| Ab No. | Primary Ab | Host | Dilution | Pretreatment | Ab source | Detection | Comments |
|---|---|---|---|---|---|---|---|
| 1510/1513 | MERS-CoV | Mouse hyperimmune serum | 1:200 | PK | CDC, DVD | (no. 1253) UVLP | MERS-CoV_JordanN3 |
| 1511 | EMC S | Mouse hyperimmune serum | 1:500 | PK | Ralph Baric, UNC | (no. 1253) UVLP | |
| 1512 | HKU5.2 N | Mouse hyperimmune serum | 1:500 | AR | CDC, DVD | (no. 1253) UVLP | Bat coronavirus |
| 1514 | γ-Irradiated IN case serum | Human | 1:1000 | AR | CDC, DVD | (no. 1518) Klear | |
| 0274 | CD68 (KP1) | MAB | 1:100 | AR | Dako | (no. 1371) Envision Doublestain | |
| 0304 | Cytokeratin (AE1 and AE3) | MAB | 1:100 | AR | Dako | (no. 1371) Envision Doublestain | |
| 1037 | Surfactant apoprotein A (PE10) | HMAF | 1:100 | AR | Dako | (no. 1371) Envision Doublestain | |
| 1547 | DPP4 (clone OTI11D7) | MAB | 1:100 | PK | Origene Technologies | (no. 1371) Envision Doublestain |
Ab, antibody; AR, antigen retrieval; DPP4, dipeptidyl peptidase 4; DVD, Division of Viral Diseases (CDC); EMC S, spike protein of human coronavirus Erasmus Medical Center; Envision Doublestain, Envision G/2 Double Stain System, Rabbit/Mouse (DAB + Permanent Red); HKU5.2 N, bat coronavirus HKU5.2 nucleoprotein; HMAF, hyperimmune mouse ascitic fluid; Klear, Klear Human AP-Polymer Detection Kit with GBI Permanent Red Chromogen; MAB, monoclonal antibody; MERS-CoV, Middle East respiratory syndrome coronavirus; PK, proteinase K; UNC, University of North Carolina; UVLP, UltraVision Labeled Polymer Detection Kit.