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. 2020 Mar 18;8:166. doi: 10.3389/fcell.2020.00166

FIGURE 1.

FIGURE 1

Co-culture platforms. (A) Tumor organoids as well as cultures of different non-neoplastic cell types can be established from the same patient and used to model cell-to-cell interactions. (B) Classical co-culture platform in which organoids and CAFs are embedded in the same drop of Matrigel®. (C) Transwell co-culture system in which organoids are embedded in a matrix on top of a transwell insert, CAFs are plated as monolayer culture. (D) Organoids-CAFs aggregates embedded in a drop of Matrigel®. (E) Organoids-CAFs aggregates embedded in a laminin-rich matrix on a Matrigel® precoated well. (F) Co-culture of organoids and peripheral blood lymphocytes, the organoids are embedded in a drop of Geltrex® while the lymphocytes are in suspension in the culture media. (G) Air-liquid interface co-culture system, patient-derived macrophages, T cells, CAFs, and cancer cells are embedded in collagen type I on top of a permeable insert precoated with collagen matrix. CAFs, cancer-associated fibroblasts.