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. 2019 Dec 17;71(6):2085–2097. doi: 10.1093/jxb/erz556

Figure 7.

Figure 7.

Downstream MAPKs of the MKD1-dependent signaling cascade in response to T-2 toxin. (A) MAP kinase activities were investigated in mock- or T-2 toxin-treated WT and mkd1 mutant plants by in-gel kinase assays. Similar results were obtained in two independent experiments. (B) Immunoprecipitation kinase assay carried out with T-2 toxin-treated WT and mkd1 mutant plants using an anti-MPK6 antibody. Similar results were obtained in two independent experiments. (C) p44 and p47 MAPK correspond to MPK3 and MPK6, respectively. MAP kinase activities were examined in WT, mpk3, and mpk6 mutant plants after 3h of T-2 toxin treatment. (D) Activation of MPK3 and MPK6 by T-2 toxin was suppressed in the mkd1 mutant. These MAPK activities were investigated in WT and mkd1 mutant plants after 3 h of T-2 toxin treatment by in-gel kinase assays (n=3). Then, corresponding bands were quantified. *P<0.05, **P<0.01, based on Student’s t-test.